This service is currently free of charge, too. If you are sorting in the CFFC you will receive step-by-step tuition and guidance leading you to being able to work with the FACSAria sorter independently and operator-free. This workshop is co-organized by TransMed and the Core Facility Flow Cytometry (CFFC)Ībout the Core Facility Flow Cytometry (CFFC)Įveryone is welcome to use FlowJo TM on the CFFC workstations, currently free of charge. Please bring your own data with you in order to analyze them.įor the workshop 1 CP for scientific skills training can be credited (0.5 CP for participating only in the theoretical part). Please read the FlowJo TM description, which will be sent to you after your registration. Theoretical and practical input by the trainer, showing examples, analyzing own data using FlowJo TM with the help of the trainer, discussing own issues, feedback from the trainer.ĭoctoral students, postdocs and clinician scientists who already know the basics of FlowJo TM. Using R-independent plugins with FlowJo TM.Using R-dependent plugins with FlowJo TM.Editing an existing compensation matrix.Creating a compensation matrix using the Wizard.Heat mapping statistics in the Table and Layout Editors.Overlays - grouping by panel or keyword.General overview of FlowJo TM including the following: A portal license for the flow cytometry analysis software FlowJo can be requested by writing to.There is enough time to discuss specific issues. In the afternoon, participants practice FlowJo in pairs using own data. In the morning, the software is explained theoretically using examples. The cost for each registration is billed to the registrants’ labs through iLab.This beyond the basic workshop shows participants how to analyze flow cytometry data using FlowJo TM. The year is broken into quarters and costs are pro-rated, so a registration active for only 6 months counts as 0.5 seats that year. The more seats we purchase, the less each seat costs.Įach spring, the Flow Lab looks at the seats that were active on the FlowJo site license for any part of the previous year. The Flow Lab pays FlowJo in advance for all the active registrations (called “seats”) on our site license, and adds additional seats as needed during the year to fill requests from researchers on campus. When the next round of software billing is completed (spring of each year), the lab will receive one more bill for the fraction of the year that seat was active. Once a seat is deactivated, it will accrue no new charges. Email the Flow Lab to request deactivation of accounts/computers as needed. Frequency of use does not matter, nor does the lab affiliation of the registered user (for an Individual User Registration) or the retirement of the computer (for a Shared Computer Registration). Deactivating a FlowJo License Registration:Ī registration on the site license remains active until the Flow Lab receives a request to deactivate the registration. Please fill out this form to request a seat on the FlowJo Site License. Id like to know how to calculate the mean of fluorescence per cell by flow cytometry. This option works well for a shared workstation used by multiple lab members.Īctivating a FlowJo License Registration: Mean of fluorescence in FlowJo - (Apr/18/2014 ). The license is only valid for that one computer, but anyone with a UW email who wants to use that workstation can make a (free) Portal account to log in and analyze data. Shared Computer Registration: Software authentication is tied to the hardware address of a single computer.It is intended to be used by only one person. This allows flexibility to use FlowJo on multiple workstations both on and off campus. The registered user can install FlowJo on up to 4 computers, and log in to one computer at a time to use the software. Rather, cell-mediated immunity is the activation of phagocytes, antigen-specific cytotoxic T-lymphocytes, and the release of various cytokines in response to an antigen. Cell-mediated immunity or cellular immunity is an immune response that does not involve antibodies.
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